Human PREX1 ELISA Kit

Product Name:Human PREX1 ELISA Kit
Packing:96T

Catalog No.:ELA-E3184h

Gene Name:PREX1

Detect Range:0.156-10 ng/mL

Sensitivity:0.082ng/mL

Target Protein Name:Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein

Alternative Name:Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3, 4, 5)-dependent Rac exchanger 1, PREX1, KIAA1415
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PREX1 ELISA Kit allows for the in vitro quantitative determination of Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PREX1 ELISA Kit has been pre-coated with an PREX1 antibody specific to Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!