Human PRKACB ELISA Kit

Product Name:Human PRKACB ELISA Kit
Packing:96T

Catalog No.:ELA-E1987h

Gene Name:PRKACB

Detect Range:78-5000 pg/mL

Sensitivity:20pg/mL

Target Protein Name:Human cAMP-dependent protein kinase catalytic subunit beta

Alternative Name:cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PRKACB ELISA Kit allows for the in vitro quantitative determination of Human cAMP-dependent protein kinase catalytic subunit beta concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PRKACB ELISA Kit has been pre-coated with an PRKACB antibody specific to Human cAMP-dependent protein kinase catalytic subunit beta .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human cAMP-dependent protein kinase catalytic subunit beta and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human cAMP-dependent protein kinase catalytic subunit beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human cAMP-dependent protein kinase catalytic subunit beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!