Human PRKCE ELISA Kit

Product Name:Human PRKCE ELISA Kit
Packing:96T

Catalog No.:ELA-E8827h

Gene Name:PRKCE

Detect Range:1.56-100 ng/mL

Sensitivity:0.98ng/mL

Target Protein Name:Human Protein kinase C epsilon type

Alternative Name:Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PRKCE ELISA Kit allows for the in vitro quantitative determination of Human Protein kinase C epsilon type concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PRKCE ELISA Kit has been pre-coated with an PRKCE antibody specific to Human Protein kinase C epsilon type .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein kinase C epsilon type and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein kinase C epsilon type, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Protein kinase C epsilon type in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!