Human ProHAMP ELISA Kit

Product Name:Human ProHAMP ELISA Kit
Packing:96T

Catalog No.:ELA-E1995h

Gene Name:ProHAMP

Detect Range:0.312-20 ng/mL

Sensitivity:0.078 ng/mL

Target Protein Name:Human Hepcidin prohormone

Alternative Name:Hepcidin prohormone, ProHAMP,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ProHAMP ELISA Kit allows for the in vitro quantitative determination of Human Hepcidin prohormone concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ProHAMP ELISA Kit has been pre-coated with an ProHAMP antibody specific to Human Hepcidin prohormone .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Hepcidin prohormone and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Hepcidin prohormone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Hepcidin prohormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!