Human Prolactin receptor, PRLR ELISA KIT
Product Name:Human Prolactin receptor, PRLR ELISA KIT
Packing:96T
Catalog No.:ELI-19730h
Gene Name:PRLR
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Prolactin receptor(PRLR
Alternative Name:PRLR,Prolactin receptor(PRLR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Prolactin receptor, PRLR ELISA KIT allows for the in vitro quantitative determination of Prolactin receptor(PRLR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Prolactin receptor, PRLR ELISA KIT has been pre-coated with an Prolactin receptor(PRLR antibody specific to Prolactin receptor(PRLR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Prolactin receptor(PRLR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prolactin receptor(PRLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Prolactin receptor(PRLR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!