Human Prominin- 1, PROM1 ELISA KIT

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human PROM1 ELISA KIT ,Human PROM1 ELISA KIT ,Human AC133 ELISA KIT ,Human CORD12 ELISA KIT ,Human MCDR2 ELISA KIT ,Human MSTP061 ELISA KIT ,Human PROML1 ELISA KIT ,Human RP41 ELISA KIT ,Human STGD4 ELISA KIT ,Human antigen AC133 ELISA KIT ,Human hProminin ELISA KIT ,Human hematopoietic stem cell antigen ELISA KIT ,Human prominin-like protein 1 ELISA KIT ,Human prominin 1 ELISA KIT

Intended use

Human PROM1 ELISA kit allows for the in vitro quantitative determination of Human PROM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human PROM1 ELISA kit has been pre-coated with an antibody specific to CD133. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD133 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CD133, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CD133 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

Human PROM1 ELISA kit Manual download

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