Human Proteasome subunit beta type- 10, PSMB10 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PSMB10,LMP10; MECL1; beta2i; low molecular mass protein 10; macropain subunit MECl-1; multicatalytic endopeptidase complex subunit MECl-1; proteasome MECl-1; proteasome catalytic subunit 2i; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta type-10; proteasome subunit beta-2i; proteasome (prosome, macropain)subunit, beta type, 10

Search name

Human PSMB10 ELISA KIT ,Human LMP10 ELISA KIT ,Human MECL1 ELISA KIT ,Human beta2i ELISA KIT ,Human low molecular mass protein 10 ELISA KIT ,Human macropain subunit MECl-1 ELISA KIT ,Human multicatalytic endopeptidase complex subunit MECl-1 ELISA KIT ,Human proteasome MECl-1 ELISA KIT ,Human proteasome catalytic subunit 2i ELISA KIT ,Human proteasome subunit MECL1 ELISA KIT ,Human proteasome subunit beta 7i ELISA KIT ,Human proteasome subunit beta type-10 ELISA KIT ,Human proteasome subunit beta-2i ELISA KIT ,Human proteasome (prosome ELISA KIT ,Human  macropain)subunit beta type 10 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Proteasome subunit beta type- 10, PSMB10 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Proteasome subunit beta type- 10, PSMB10, During the reaction, Human Proteasome subunit beta type- 10, PSMB10 in the sample or standard competes with a fixed amount of biotin-labeled Human Proteasome subunit beta type- 10, PSMB10 for sites on a pre-coated Monoclonal antibody specific to Human Proteasome subunit beta type- 10, PSMB10. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Proteasome subunit beta type- 10, PSMB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.