Human Protein phosphatase 1B, PPM1B ELISA KIT

Product Name:Human Protein phosphatase 1B, PPM1B ELISA KIT
Packing:96T

Catalog No.:ELI-22337h

Gene Name:PPM1B

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Protein phosphatase 1B(PPM1B

Alternative Name:PPM1B,Protein phosphatase 1B(PPM1B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Protein phosphatase 1B, PPM1B ELISA KIT allows for the in vitro quantitative determination of Protein phosphatase 1B(PPM1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Protein phosphatase 1B, PPM1B ELISA KIT has been pre-coated with an Protein phosphatase 1B(PPM1B antibody specific to Protein phosphatase 1B(PPM1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein phosphatase 1B(PPM1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein phosphatase 1B(PPM1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein phosphatase 1B(PPM1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!