Human Protein phosphatase 1E, PPM1E ELISA KIT
Product Name:Human Protein phosphatase 1E, PPM1E ELISA KIT
Packing:96T
Catalog No.:ELI-15387h
Gene Name:PPM1E
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Protein phosphatase 1E(PPM1E
Alternative Name:PPM1E,Protein phosphatase 1E(PPM1E
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein phosphatase 1E, PPM1E ELISA KIT allows for the in vitro quantitative determination of Protein phosphatase 1E(PPM1E concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein phosphatase 1E, PPM1E ELISA KIT has been pre-coated with an Protein phosphatase 1E(PPM1E antibody specific to Protein phosphatase 1E(PPM1E .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein phosphatase 1E(PPM1E and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein phosphatase 1E(PPM1E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein phosphatase 1E(PPM1E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!