Human Protein Phosphatase ELISA Kit

Product Name:Human Protein Phosphatase ELISA Kit
Packing:96T

Catalog No.:ELA-E0268h

Gene Name:PP

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Protein Phosphatase

Alternative Name:PP,Protein Phosphatase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Protein Phosphatase ELISA Kit allows for the in vitro quantitative determination of Protein Phosphatase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Protein Phosphatase ELISA Kit has been pre-coated with an Protein Phosphatase antibody specific to Protein Phosphatase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein Phosphatase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein Phosphatase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!