Human Proto- oncogene Wnt- 3, WNT3 ELISA KIT
Product Name:Human Proto- oncogene Wnt- 3, WNT3 ELISA KIT
Packing:96T
Catalog No.:ELI-28275h
Gene Name:WNT3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Proto-oncogene Wnt-3(WNT3
Alternative Name:WNT3,Proto-oncogene Wnt-3(WNT3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proto- oncogene Wnt- 3, WNT3 ELISA KIT allows for the in vitro quantitative determination of Proto-oncogene Wnt-3(WNT3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proto- oncogene Wnt- 3, WNT3 ELISA KIT has been pre-coated with an Proto-oncogene Wnt-3(WNT3 antibody specific to Proto-oncogene Wnt-3(WNT3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proto-oncogene Wnt-3(WNT3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proto-oncogene Wnt-3(WNT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proto-oncogene Wnt-3(WNT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!