Human PRPSAP1 ELISA Kit

Product Name:Human PRPSAP1 ELISA Kit
Packing:96T

Catalog No.:ELA-E13233h

Gene Name:PRPSAP1

Detect Range:0.156-10 ng/mL

Sensitivity:0.1ng/ml

Target Protein Name:Human Phosphoribosyl pyrophosphate synthase-associated protein 1

Alternative Name:Phosphoribosyl pyrophosphate synthase-associated protein 1, PRPP synthase-associated protein 1, 39 kDa phosphoribosypyrophosphate synthase-associated protein, PAP39, PRPSAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PRPSAP1 ELISA Kit allows for the in vitro quantitative determination of Human Phosphoribosyl pyrophosphate synthase-associated protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PRPSAP1 ELISA Kit has been pre-coated with an PRPSAP1 antibody specific to Human Phosphoribosyl pyrophosphate synthase-associated protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Phosphoribosyl pyrophosphate synthase-associated protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phosphoribosyl pyrophosphate synthase-associated protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Phosphoribosyl pyrophosphate synthase-associated protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!