Human Rapamycin- insensitive companion of mTOR, RICTOR ELISA KIT

Product Name:Human Rapamycin- insensitive companion of mTOR, RICTOR ELISA KIT
Packing:96T

Catalog No.:ELI-18108h

Gene Name:RICTOR

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Rapamycin-insensitive companion of mTOR(RICTOR

Alternative Name:RICTOR,Rapamycin-insensitive companion of mTOR(RICTOR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Rapamycin- insensitive companion of mTOR, RICTOR ELISA KIT allows for the in vitro quantitative determination of Rapamycin-insensitive companion of mTOR(RICTOR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Rapamycin- insensitive companion of mTOR, RICTOR ELISA KIT has been pre-coated with an Rapamycin-insensitive companion of mTOR(RICTOR antibody specific to Rapamycin-insensitive companion of mTOR(RICTOR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rapamycin-insensitive companion of mTOR(RICTOR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rapamycin-insensitive companion of mTOR(RICTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rapamycin-insensitive companion of mTOR(RICTOR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!