Human Regulator of G-protein signaling 3, RGS3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

RGS3,C2PA; RGP3; C2PA,regulator of G-protein signalling 3; regulator of G-protein signaling 3

Search name

Human RGS3 ELISA KIT ,Human C2PA ELISA KIT ,Human RGP3 ELISA KIT ,Human C2PA ELISA KIT ,Human regulator of G-protein signalling 3 ELISA KIT ,Human regulator of G-protein signaling 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human regulator of g-protein signaling 3,RGS3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to RGS3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RGS3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RGS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RGS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.