Human Replication protein A 32 kDa subunit, RPA2 ELISA KIT

Product Name:Human Replication protein A 32 kDa subunit, RPA2 ELISA KIT
Packing:96T

Catalog No.:ELI-18078h

Gene Name:RPA2

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Replication protein A 32 kDa subunit(RPA2

Alternative Name:RPA2,Replication protein A 32 kDa subunit(RPA2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Replication protein A 32 kDa subunit, RPA2 ELISA KIT allows for the in vitro quantitative determination of Replication protein A 32 kDa subunit(RPA2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Replication protein A 32 kDa subunit, RPA2 ELISA KIT has been pre-coated with an Replication protein A 32 kDa subunit(RPA2 antibody specific to Replication protein A 32 kDa subunit(RPA2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Replication protein A 32 kDa subunit(RPA2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Replication protein A 32 kDa subunit(RPA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Replication protein A 32 kDa subunit(RPA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!