Human Rho GTPase- activating protein 23, ARHGAP23 ELISA KIT
Product Name:Human Rho GTPase- activating protein 23, ARHGAP23 ELISA KIT
Packing:96T
Catalog No.:ELI-35576h
Gene Name:ARHGAP23
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rho GTPase-activating protein 23(ARHGAP23
Alternative Name:ARHGAP23,Rho GTPase-activating protein 23(ARHGAP23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 23, ARHGAP23 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 23(ARHGAP23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 23, ARHGAP23 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 23(ARHGAP23 antibody specific to Rho GTPase-activating protein 23(ARHGAP23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 23(ARHGAP23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 23(ARHGAP23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 23(ARHGAP23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!