Human Ribonuclease 8, RNASE8 ELISA KIT

Product Name:Human Ribonuclease 8, RNASE8 ELISA KIT
Packing:96T

Catalog No.:ELI-42654h

Gene Name:RNASE8

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Ribonuclease 8(RNASE8

Alternative Name:RNASE8,Ribonuclease 8(RNASE8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Ribonuclease 8, RNASE8 ELISA KIT allows for the in vitro quantitative determination of Ribonuclease 8(RNASE8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Ribonuclease 8, RNASE8 ELISA KIT has been pre-coated with an Ribonuclease 8(RNASE8 antibody specific to Ribonuclease 8(RNASE8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ribonuclease 8(RNASE8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ribonuclease 8(RNASE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ribonuclease 8(RNASE8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!