Human Ribosome biogenesis protein WDR12,WDR12 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

WDR12,ribosome biogenesis protein WDR12; YTM1; WD repeat-containing protein 12; WD repeat domain 12

Search name

Human WDR12 ELISA KIT ,Human ribosome biogenesis protein WDR12 ELISA KIT ,Human YTM1 ELISA KIT ,Human WD repeat-containing protein 12 ELISA KIT ,Human WD repeat domain 12 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Ribosome biogenesis protein WDR12,WDR12 concentrations in serum,plasma,tissue homogenates,cell culture supernates,and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ribosome biogenesis protein WDR12,WDR12,During the reaction,Human Ribosome biogenesis protein WDR12,WDR12 in the sample or standard competes with a fixed amount of biotin-labeled Human Ribosome biogenesis protein WDR12,WDR12 for sites on a pre-coated Monoclonal antibody specific to Human Ribosome biogenesis protein WDR12,WDR12. Excess conjugate and unbound sample or standard are washed from the plate. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ribosome biogenesis protein WDR12,WDR12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.