Human RING finger protein 114, RNF114 ELISA KIT

Product Name:Human RING finger protein 114, RNF114 ELISA KIT
Packing:96T

Catalog No.:ELI-41378h

Gene Name:RNF114

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:RING finger protein 114(RNF114

Alternative Name:RNF114,RING finger protein 114(RNF114
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human RING finger protein 114, RNF114 ELISA KIT allows for the in vitro quantitative determination of RING finger protein 114(RNF114 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human RING finger protein 114, RNF114 ELISA KIT has been pre-coated with an RING finger protein 114(RNF114 antibody specific to RING finger protein 114(RNF114 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RING finger protein 114(RNF114 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RING finger protein 114(RNF114, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of RING finger protein 114(RNF114 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!