Human RNASE2 ELISA Kit
Product Name:Human RNASE2 ELISA Kit
Packing:96T
Catalog No.:ELA-E0765h
Gene Name:RNASE2
Detect Range:6.25-400 U/mL
Sensitivity:1.8 U/mL
Target Protein Name:Human Non-secretory ribonuclease
Alternative Name:Non-secretory ribonuclease, Eosinophil-derived neurotoxin, RNase UpI-2, Ribonuclease 2, RNase 2, Ribonuclease US, RNASE2, EDN, RNS2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human RNASE2 ELISA Kit allows for the in vitro quantitative determination of Human Non-secretory ribonuclease concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human RNASE2 ELISA Kit has been pre-coated with an RNASE2 antibody specific to Human Non-secretory ribonuclease .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Non-secretory ribonuclease and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Non-secretory ribonuclease, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm