Human ROCK2 ELISA Kit
Product Name:Human ROCK2 ELISA Kit
Packing:96T
Catalog No.:ELA-E1852h
Gene Name:ROCK2
Detect Range:1.56-100 ng/mL
Sensitivity:0.76ng/mL
Target Protein Name:Human Rho-associated protein kinase 2
Alternative Name:Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2, ROCK2,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ROCK2 ELISA Kit allows for the in vitro quantitative determination of Human Rho-associated protein kinase 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ROCK2 ELISA Kit has been pre-coated with an ROCK2 antibody specific to Human Rho-associated protein kinase 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Rho-associated protein kinase 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Rho-associated protein kinase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Rho-associated protein kinase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!