Human RYR1 ELISA Kit
Product Name:Human RYR1 ELISA Kit
Packing:96T
Catalog No.:ELA-E1435h
Gene Name:RYR1
Detect Range:0.156-10 ng/mL
Sensitivity:0.067ng/mL
Target Protein Name:Human Ryanodine receptor 1
Alternative Name:Ryanodine receptor 1, RYR-1, RyR1, Skeletal muscle calcium release channel, Skeletal muscle-type ryanodine receptor, RYR1, RYDR, Skeletal muscle-type ryanodine receptor, Type 1 ryanodine
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human RYR1 ELISA Kit allows for the in vitro quantitative determination of Human Ryanodine receptor 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human RYR1 ELISA Kit has been pre-coated with an RYR1 antibody specific to Human Ryanodine receptor 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ryanodine receptor 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ryanodine receptor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm