Human Sarcosine dehydrogenase mitochondrial, SarDH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SarDH,BPR-2, sarcosine dehydrogenase,BPR-2; DMGDHL1; SAR; SARD; SDH; dimethylglycine dehydrogenase-like 1; sarcosine dehydrogenase mitochondrial

Search name

Human SarDH ELISA KIT ,Human BPR-2 ELISA KIT ,Human  sarcosine dehydrogenase ELISA KIT ,Human BPR-2 ELISA KIT ,Human DMGDHL1 ELISA KIT ,Human SAR ELISA KIT ,Human SARD ELISA KIT ,Human SDH ELISA KIT ,Human dimethylglycine dehydrogenase-like 1 ELISA KIT ,Human sarcosine dehydrogenase mitochondrial ELISA KIT

Intended use

Human SarDH ELISA KIT allows for the in vitro quantitative determination of human sarcosine dehydrogenase, mitochondrial,SarDH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human SarDH ELISA KIT has been pre-coated with an antibody specific to SarDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SarDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SarDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SarDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human SarDH ELISA KIT PDF Manual download