Human SELS ELISA Kit
Product Name:Human SELS ELISA Kit
Packing:96T
Catalog No.:ELA-E10141h
Gene Name:SELS
Detect Range:31.2-2000 pg/mL
Sensitivity:17pg/mL
Target Protein Name:Human Selenoprotein S
Alternative Name:Selenoprotein S, SelS, VCP-interacting membrane protein, SELS, VIMP, AD-015, SBBI8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SELS ELISA Kit allows for the in vitro quantitative determination of Human Selenoprotein S concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SELS ELISA Kit has been pre-coated with an SELS antibody specific to Human Selenoprotein S .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Selenoprotein S and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Selenoprotein S, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Selenoprotein S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!