Human Semaphorin- 4F, SEMA4F ELISA KIT

Product Name:Human Semaphorin- 4F, SEMA4F ELISA KIT
Packing:96T

Catalog No.:ELI-19191h

Gene Name:SEMA4F

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Semaphorin-4F(SEMA4F

Alternative Name:SEMA4F,Semaphorin-4F(SEMA4F
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Semaphorin- 4F, SEMA4F ELISA KIT allows for the in vitro quantitative determination of Semaphorin-4F(SEMA4F concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Semaphorin- 4F, SEMA4F ELISA KIT has been pre-coated with an Semaphorin-4F(SEMA4F antibody specific to Semaphorin-4F(SEMA4F .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Semaphorin-4F(SEMA4F and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Semaphorin-4F(SEMA4F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Semaphorin-4F(SEMA4F in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!