Human Serpin B13, SERPINB13 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Serpin B13,Proteinase inhibitor 13,Peptidase inhibitor 13(PI-13),HaCaT UV-repressible serpin(Hurpin),Headpin,SERPINB13,serpin B13; HSHUR7SEQ; HUR7; PI13; headpin; HURPIN; PI-13; UV-B repressed sequence, HUR 7; haCaT UV-repressible serpin; peptidase inhibitor 13; protease inhibitor 13 hurpin headpin; proteinase inhibitor 13; serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 13; serpin peptidase inhibitor, clade B (ovalbumin) member 13

Search name

Human Serpin B13 ELISA KIT ,Human Proteinase inhibitor 13 ELISA KIT ,Human Peptidase inhibitor 1 ELISA KIT ,Human PI-13 ELISA KIT ,Human HaCaT UV-repressible serpin(Hurpin) ELISA KIT ,Human Headpin ELISA KIT ,Human SERPINB13 ELISA KIT ,Human serpin B13 ELISA KIT ,Human HSHUR7SEQ ELISA KIT ,Human HUR7 ELISA KIT ,Human PI13 ELISA KIT ,Human headpin ELISA KIT ,Human HURPIN ELISA KIT ,Human PI-13 ELISA KIT ,Human UV-B repressed sequence ELISA KIT ,Human HUR 7 ELISA KIT ,Human haCaT UV-repressible serpin ELISA KIT ,Human peptidase inhibitor 13 ELISA KIT ,Human protease inhibitor 13 hurpin headpin ELISA KIT ,Human proteinase inhibitor 13 ELISA KIT ,Human serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 13 ELISA KIT ,Human serpin peptidase inhibitor clade B (ovalbumin) member 13 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Serpin B13 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serpin B13, During the reaction, Serpin B13 in the sample or standard competes with a fixed amount of biotin-labeled Serpin B13 for sites on a pre-coated Monoclonal antibody specific to Serpin B13. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serpin B13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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