Human SERPINA1 ELISA Kit

Product Name:Human SERPINA1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1697h

Gene Name:SERPINA1

Detect Range:3.12-200 ng/mL

Sensitivity:1.2ng/mL

Target Protein Name:Human Alpha-1-antitrypsin

Alternative Name:Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, SERPINA1, AAT, PI, PRO0684, PRO2209
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SERPINA1 ELISA Kit allows for the in vitro quantitative determination of Human Alpha-1-antitrypsin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SERPINA1 ELISA Kit has been pre-coated with an SERPINA1 antibody specific to Human Alpha-1-antitrypsin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Alpha-1-antitrypsin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Alpha-1-antitrypsin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm