Human Serum amyloid A- 4 protein, SAA4 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Serum amyloid A-4 protein,Constitutively expressed serum amyloid A protein,SAA4,C-SAA; CSAA; constitutively expressed serum amyloid A protein; serum amyloid A-4 protein; serum amyloid A4 constitutive

Search name

Human Serum amyloid A-4 protein ELISA KIT ,Human Constitutively expressed serum amyloid A protein ELISA KIT ,Human SAA4 ELISA KIT ,Human C-SAA ELISA KIT ,Human CSAA ELISA KIT ,Human constitutively expressed serum amyloid A protein ELISA KIT ,Human serum amyloid A-4 protein ELISA KIT ,Human serum amyloid A4 constitutive ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human serum amyloid a-4 protein,SAA4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SAA4 and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SAA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SAA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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