Human Serum paraoxonase/arylesterase 2, PON2 ELISA KIT

Product Name:Human Serum paraoxonase/arylesterase 2, PON2 ELISA KIT
Packing:96T

Catalog No.:ELI-36179h

Gene Name:PON2

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Serum paraoxonase(arylesterase 2(PON2

Alternative Name:PON2,Serum paraoxonase(arylesterase 2(PON2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Serum paraoxonase/arylesterase 2, PON2 ELISA KIT allows for the in vitro quantitative determination of Serum paraoxonase(arylesterase 2(PON2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Serum paraoxonase/arylesterase 2, PON2 ELISA KIT has been pre-coated with an Serum paraoxonase(arylesterase 2(PON2 antibody specific to Serum paraoxonase(arylesterase 2(PON2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Serum paraoxonase(arylesterase 2(PON2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serum paraoxonase(arylesterase 2(PON2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Serum paraoxonase(arylesterase 2(PON2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!