Human SFN ELISA Kit

Product Name:Human SFN ELISA Kit
Packing:96T

Catalog No.:ELA-E15106h

Gene Name:SFN

Detect Range:78-5000 pg/mL

Sensitivity:18 pg/ml

Target Protein Name:Human 14-3-3 protein sigma

Alternative Name:14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SFN ELISA Kit allows for the in vitro quantitative determination of Human 14-3-3 protein sigma concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SFN ELISA Kit has been pre-coated with an SFN antibody specific to Human 14-3-3 protein sigma .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human 14-3-3 protein sigma and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 14-3-3 protein sigma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human 14-3-3 protein sigma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!