Human SFRP4 ELISA Kit

Product Name:Human SFRP4 ELISA Kit
Packing:96T

Catalog No.:ELA-E1650h

Gene Name:SFRP4

Detect Range:0.156-10 ng/mL

Sensitivity:0.076ng/mL

Target Protein Name:Human Secreted frizzled-related protein 4

Alternative Name:Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SFRP4 ELISA Kit allows for the in vitro quantitative determination of Human Secreted frizzled-related protein 4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SFRP4 ELISA Kit has been pre-coated with an SFRP4 antibody specific to Human Secreted frizzled-related protein 4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Secreted frizzled-related protein 4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Secreted frizzled-related protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm