Human SH3 domain- binding protein 5- like, SH3BP5L ELISA KIT
Product Name:Human SH3 domain- binding protein 5- like, SH3BP5L ELISA KIT
Packing:96T
Catalog No.:ELI-12248h
Gene Name:SH3BP5L
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:SH3 domain-binding protein 5-like(SH3BP5L
Alternative Name:SH3BP5L,SH3 domain-binding protein 5-like(SH3BP5L
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SH3 domain- binding protein 5- like, SH3BP5L ELISA KIT allows for the in vitro quantitative determination of SH3 domain-binding protein 5-like(SH3BP5L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SH3 domain- binding protein 5- like, SH3BP5L ELISA KIT has been pre-coated with an SH3 domain-binding protein 5-like(SH3BP5L antibody specific to SH3 domain-binding protein 5-like(SH3BP5L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SH3 domain-binding protein 5-like(SH3BP5L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SH3 domain-binding protein 5-like(SH3BP5L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of SH3 domain-binding protein 5-like(SH3BP5L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!