Human SIRT7 ELISA Kit

Product Name:Human SIRT7 ELISA Kit
Packing:96T

Catalog No.:ELA-E11385h

Gene Name:SIRT7

Detect Range:0.312-20 ng/mL

Sensitivity:0.1ng/ml

Target Protein Name:Human NAD-dependent deacetylase sirtuin-7

Alternative Name:NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7, SIRT7, SIR2L7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SIRT7 ELISA Kit allows for the in vitro quantitative determination of Human NAD-dependent deacetylase sirtuin-7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SIRT7 ELISA Kit has been pre-coated with an SIRT7 antibody specific to Human NAD-dependent deacetylase sirtuin-7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NAD-dependent deacetylase sirtuin-7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NAD-dependent deacetylase sirtuin-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human NAD-dependent deacetylase sirtuin-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!