Human SMAD3 ELISA Kit

Product Name:Human SMAD3 ELISA Kit
Packing:96T

Catalog No.:ELA-E2185h

Gene Name:SMAD3

Detect Range:0.625-40 ng/mL

Sensitivity:0.159 ng/mL

Target Protein Name:Human Mothers against decapentaplegic homolog 3

Alternative Name:Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SMAD3 ELISA Kit allows for the in vitro quantitative determination of Human Mothers against decapentaplegic homolog 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SMAD3 ELISA Kit has been pre-coated with an SMAD3 antibody specific to Human Mothers against decapentaplegic homolog 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Mothers against decapentaplegic homolog 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Mothers against decapentaplegic homolog 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Mothers against decapentaplegic homolog 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!