Human Small proline- rich protein 2E, SPRR2E ELISA KIT
Product Name:Human Small proline- rich protein 2E, SPRR2E ELISA KIT
Packing:96T
Catalog No.:ELI-18272h
Gene Name:SPRR2E
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Small proline-rich protein 2E(SPRR2E
Alternative Name:SPRR2E,Small proline-rich protein 2E(SPRR2E
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Small proline- rich protein 2E, SPRR2E ELISA KIT allows for the in vitro quantitative determination of Small proline-rich protein 2E(SPRR2E concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Small proline- rich protein 2E, SPRR2E ELISA KIT has been pre-coated with an Small proline-rich protein 2E(SPRR2E antibody specific to Small proline-rich protein 2E(SPRR2E .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Small proline-rich protein 2E(SPRR2E and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Small proline-rich protein 2E(SPRR2E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Small proline-rich protein 2E(SPRR2E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!