SM/SPH ELISA KIT|Human
Catalog EF007178
Packing 48T/96T
Reactivity Human
Detect Range 3.125-200ng/ml
SM/SPH ELISA KIT Sensitivity< 1.875ng/ml
Application SM/SPH ELISA KIT|Human is for detecting the concentration of Human SM/SPH in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of SM/SPH ELISA KIT|Human
The microtiter plate provided in SM/SPH ELISA KIT|Human has been pre-coated with an Human SM/SPH antibody specific to Human SM/SPH . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human SM/SPH antibody preparation specific for Human SM/SPH then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human SM/SPH , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human SM/SPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that SM/SPH ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!