Human Soluble Vascular Endothelial Growth Factor Receptor 1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Soluble Vascular endothelial growth factor receptor 1(sVEGFR-1),;
Soluble Vascular permeability factor receptor, Soluble Tyrosine-protein kinase receptor FLT, Soluble Fms-like tyrosine kinase 1(sFLT-1),
Soluble Tyrosine-protein kinase FRT,;

Search name
Human Soluble Vascuoar endothelial cell growth factor receptor 1 ELISA kit, Human Soluble VEGFR-1 ELISA kit, Human Soluble Flt1 ELISA kit, Human Soluble Vascular permeability factor receptor ELISA kit, Human Soluble Tyrosine-protein kinase receptor FLT ELISA kit, Human Soluble Fms-like tyrosine kinase 1 ELISA kit, Human Soluble Tyrosine-protein kinase FRT ELISA kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human sVEGFR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SVEGFR-1 . Standards or samples are then
 
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SVEGFR-1
 
and
 
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain SVEGFR-1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
 
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SVEGFR-1
by comparing the O.D. of the samples to the standard curve.

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