Human Sperm surface protein Sp17, SPA17 ELISA KIT

Product Name:Human Sperm surface protein Sp17, SPA17 ELISA KIT
Packing:96T

Catalog No.:ELI-06257h

Gene Name:SPA17

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Sperm surface protein Sp17(SPA17

Alternative Name:SPA17,Sperm surface protein Sp17(SPA17
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Sperm surface protein Sp17, SPA17 ELISA KIT allows for the in vitro quantitative determination of Sperm surface protein Sp17(SPA17 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Sperm surface protein Sp17, SPA17 ELISA KIT has been pre-coated with an Sperm surface protein Sp17(SPA17 antibody specific to Sperm surface protein Sp17(SPA17 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sperm surface protein Sp17(SPA17 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sperm surface protein Sp17(SPA17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sperm surface protein Sp17(SPA17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!