Human Spondin-1, SPON1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Spon1,VSGP/F-spondin; f-spondin; spondin 1,(f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor; spondin 1, extracellular matrix protein

Search name

Human Spon1 ELISA KIT ,Human  spondin-1 ELISA KIT ,Human VSGP/F-spondin ELISA KIT ,Human f-spondin ELISA KIT ,Human spondin 1(f-spondin) extracellular matrix protein ELISA KIT ,Human vascular smooth muscle cell growth-promoting factor ELISA KIT ,Human spondin 1 ELISA KIT ,Human  extracellular matrix protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Spondin-1, SPON1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Spondin-1, SPON1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Spondin-1, SPON1 and Avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Spondin-1, SPON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Spondin-1, SPON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product

Delivery time 4-6month