Human STEAP1 ELISA Kit
Product Name:Human STEAP1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0519h
Gene Name:STEAP1
Detect Range:0.39-25 ng/mL
Sensitivity:0.21ng/mL
Target Protein Name:Human Metalloreductase STEAP1
Alternative Name:Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1, STEAP1, PRSS24, STEAP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human STEAP1 ELISA Kit allows for the in vitro quantitative determination of Human Metalloreductase STEAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human STEAP1 ELISA Kit has been pre-coated with an STEAP1 antibody specific to Human Metalloreductase STEAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Metalloreductase STEAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Metalloreductase STEAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm