Human STH ELISA Kit
Product Name:Human STH ELISA Kit
Packing:96T
Catalog No.:ELA-E0304h
Gene Name:STH
Detect Range:0.156-10 ng/mL
Sensitivity:0.067ng/mL
Target Protein Name:Human Saitohin
Alternative Name:Saitohin, STH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human STH ELISA Kit allows for the in vitro quantitative determination of Human Saitohin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human STH ELISA Kit has been pre-coated with an STH antibody specific to Human Saitohin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Saitohin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Saitohin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm