Human TFPI2 ELISA Kit
Product Name:Human TFPI2 ELISA Kit
Packing:96T
Catalog No.:ELA-E1940h
Gene Name:TFPI2
Detect Range:1.56-100 ng/mL
Sensitivity:0.79ng/mL
Target Protein Name:Human Tissue factor pathway inhibitor 2
Alternative Name:Tissue factor pathway inhibitor 2, TFPI-2, Placental protein 5, PP5, TFPI2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TFPI2 ELISA Kit allows for the in vitro quantitative determination of Human Tissue factor pathway inhibitor 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TFPI2 ELISA Kit has been pre-coated with an TFPI2 antibody specific to Human Tissue factor pathway inhibitor 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Tissue factor pathway inhibitor 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Tissue factor pathway inhibitor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Tissue factor pathway inhibitor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!