Human Thiopurine S-methyltransferase, TPMT ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Thiopurine S-methyltransferase,Thiopurine methyltransferase, TPMT,S-adenosyl-L-methionine:thiopurine S-methyltransferase

Search name

Human Thiopurine S-methyltransferase ELISA KIT ,Human Thiopurine methyltransferase ELISA KIT ,Human TPMT ELISA KIT ,Human S-adenosyl-L-methionine ELISA KI, Human thiopurine S-methyltransferase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human thiopurine s-methyltransferase,TPMT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPMT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TPMT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TPMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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