Human Thioredoxin, TXN ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Thioredoxin(Trx),ATL-derived factor(ADF),Surface-associated sulphydryl protein(SASP), Thioredoxin,TRX; TRDX; TRX1; ADF;
ATL-derived factor; SASP; TXN delta 3; surface-associated sulphydryl protein; thioredoxin delta 3

Search name
Human Thioredoxin ELISA Kit, Human Trx ELISA Kit, Human ATL-derived factor ELISA Kit, Human ADF ELISA Kit, Human
Surface-associated sulphydryl protein ELISA Kit, Human SASP ELISA Kit, Human TRDX ELISA Kit, Human Trx1 ELISA Kit,
Human TXN delta 3 ELISA Kit, Human thioredoxin delta 3 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human thioredoxin,Trx concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trx. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Trx and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Trx, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Trx in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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