Human THOP1 ELISA Kit

Product Name:Human THOP1 ELISA Kit
Packing:96T

Catalog No.:ELA-E13023h

Gene Name:THOP1

Detect Range:0.312-20 ng/mL

Sensitivity:0.13ng/ml

Target Protein Name:Human Thimet oligopeptidase

Alternative Name:Thimet oligopeptidase, Endopeptidase 24.15, MP78, THOP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human THOP1 ELISA Kit allows for the in vitro quantitative determination of Human Thimet oligopeptidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human THOP1 ELISA Kit has been pre-coated with an THOP1 antibody specific to Human Thimet oligopeptidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Thimet oligopeptidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Thimet oligopeptidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Thimet oligopeptidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!