Human Thrombospondin-1, THBS1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

THBS1,thrombospondin 1,THBS; THBS-1; TSP; TSP-1; TSP1; thrombospondin-1p180; thrombospondin-p50, Thrombospondin-1

Search name

Human THBS1 ELISA KIT ,Human thrombospondin 1 ELISA KIT ,Human THBS ELISA KIT ,Human THBS-1 ELISA KIT ,Human TSP ELISA KIT ,Human TSP-1 ELISA KIT ,Human TSP1 ELISA KIT ,Human thrombospondin-1p180 ELISA KIT ,Human thrombospondin-p50 ELISA KIT ,Human Thrombospondin-1 ELISA KIT  

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Thrombospondin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombospondin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombospondin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombospondin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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