Human TK2 ELISA Kit

Product Name:Human TK2 ELISA Kit
Packing:96T

Catalog No.:ELA-E2367h

Gene Name:TK2

Detect Range:0.78-50 ng/mL

Sensitivity:0.37ng/mL

Target Protein Name:Human Thymidine kinase 2, mitochondrial

Alternative Name:Thymidine kinase 2, mitochondrial, Mt-TK, TK2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human TK2 ELISA Kit allows for the in vitro quantitative determination of Human Thymidine kinase 2, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human TK2 ELISA Kit has been pre-coated with an TK2 antibody specific to Human Thymidine kinase 2, mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Thymidine kinase 2, mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Thymidine kinase 2, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Thymidine kinase 2, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!