Human TPSG1 ELISA Kit
Product Name:Human TPSG1 ELISA Kit
Packing:96T
Catalog No.:ELA-E10501h
Gene Name:TPSG1
Detect Range:31.2-2000 pg/mL
Sensitivity:10pg/mL
Target Protein Name:Human Tryptase gamma
Alternative Name:Tryptase gamma, Serine protease 31, Transmembrane tryptase, TPSG1, PRSS31, TMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TPSG1 ELISA Kit allows for the in vitro quantitative determination of Human Tryptase gamma concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TPSG1 ELISA Kit has been pre-coated with an TPSG1 antibody specific to Human Tryptase gamma .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Tryptase gamma and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Tryptase gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Tryptase gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!