Human Trans- 2,3- enoyl- CoA reductase, TECR ELISA KIT
Product Name:Human Trans- 2,3- enoyl- CoA reductase, TECR ELISA KIT
Packing:96T
Catalog No.:ELI-37089h
Gene Name:TECR
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Trans-2,3-enoyl-CoA reductase(TECR
Alternative Name:TECR,Trans-2,3-enoyl-CoA reductase(TECR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Trans- 2,3- enoyl- CoA reductase, TECR ELISA KIT allows for the in vitro quantitative determination of Trans-2,3-enoyl-CoA reductase(TECR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Trans- 2,3- enoyl- CoA reductase, TECR ELISA KIT has been pre-coated with an Trans-2,3-enoyl-CoA reductase(TECR antibody specific to Trans-2,3-enoyl-CoA reductase(TECR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Trans-2,3-enoyl-CoA reductase(TECR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Trans-2,3-enoyl-CoA reductase(TECR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Trans-2,3-enoyl-CoA reductase(TECR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!