Human Transcription factor AP- 2- delta, TFAP2D ELISA KIT
Product Name:Human Transcription factor AP- 2- delta, TFAP2D ELISA KIT
Packing:96T
Catalog No.:ELI-23874h
Gene Name:TFAP2D
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Transcription factor AP-2-delta(TFAP2D
Alternative Name:TFAP2D,Transcription factor AP-2-delta(TFAP2D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Transcription factor AP- 2- delta, TFAP2D ELISA KIT allows for the in vitro quantitative determination of Transcription factor AP-2-delta(TFAP2D concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Transcription factor AP- 2- delta, TFAP2D ELISA KIT has been pre-coated with an Transcription factor AP-2-delta(TFAP2D antibody specific to Transcription factor AP-2-delta(TFAP2D .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transcription factor AP-2-delta(TFAP2D and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor AP-2-delta(TFAP2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transcription factor AP-2-delta(TFAP2D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!